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• Cell Lab Quanta SC Instructions for Use PN 721742AD (October 2011) Manufactured for Beckman Coulter, Inc. Kraemer Blvd. Brea, CA 92821. • This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals. R You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s computer with software authorized by Beckman Coulter.

• This document applies to the latest software listed and higher versions. When a subsequent software version affects the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or system. • REVISION STATUS PN 721742AD.

• CONTENTS REVISION STATUS, iii CONTENTS, v Illustrations, xi Tables, xiii INTRODUCTION, xv Text Conventions, xvi Graphic Conventions, xvi Safety Symbols, xvii USE AND FUNCTION, 1-1 SYSTEM OVERVIEW, 1-1 INTENDED USE, 1-1 QUANTA SC SYSTEM OVERVIEW, 1-2 SYSTEM COMPONENTS, 1-3 PRINCIPLES OF OPERATION, 1-4 WARNING LABELS AND PRECAUTIONS, 1-5. • CONTENTS Electrical Input, 2-3 Ambient Temperature and Humidity, 2-3 Heat Dissipation, 2-3 Drainage, 2-4 INSTALLATION PROCEDURES, 2-4 Interunit Connections, 2-4 Lifting and Carrying, 2-4 OPERATION PRINCIPLES, 3-1 OVERVIEW, 3-1 SAMPLE FLOW, 3-1 SPECIFICATIONS, 4-1 PERFORMANCE SPECIFICATIONS, 4-1 Absolute Count, 4-1 Fluorescence, 4-1 Sizing, 4-1 Optics, 4-1. • CONTENTS Menu Options, 5-4 File Menu, 5-5 Instrument Menu, 5-5 Gain Menu, 5-6 Volume Menu, 5-6 Analysis Menu, 5-6 Regions Screen, 5-7 Compensation Screen, 5-7 Protocols Screen, 5-8 Current Settings Screen, 5-8 File Information Screen, 5-9 Security Menu, 5-9 Help Menu, 5-10 Closing a Window or Closing the Software, 5-10.